Composite

Part:BBa_K612002:Design

Designed by: Michael Simpson   Group: iGEM11_Alberta   (2011-09-28)

TesA' Whole Gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1675
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1187
    Illegal BsaI.rc site found at 1695


Design Notes

This part is used in conjunction with the 5'FadD-UTR (BBa_K612001) the 3'FadD-UTR (BBa_K612004) and Hygromycin B gene (BBa_K612003) in order to knockout the FadD gene while inserting the TesA' gene into N.Crassa. The FadD-UTR's allow for homologous recombination so that the gene replacement can take place. The Hygromycin B is the selection marker for the transformation.

Source

This was synthesized by IDT.

References